PiRV-2 stimulates sporulation in Phytophthora infestans

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[Heteroplasmosis in Phytophthora infestans].

APCR and monozoospore plating was used to demonstrate a simultaneous presence of the mitochondria with mitochondrial DNA of haplotypes ILa and IIa in the mycelium of several Phytophthora infestans strains.

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Life cycle progression in eukaryotic microbes is often influenced by environment. In the oomycete Phytophthora infestans, which causes late blight on potato and tomato, sporangia have been reported to form mostly at night. By growing P. infestans under different light regimes at constant temperature and humidity, we show that light contributes to the natural pattern of sporulation by delaying s...

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The Cdc14 gene of Phytophthora infestans is transcribed specifically during sporulation, with no mRNA detectable in vegetative hyphae, and is required for sporangium development. To unravel the mechanisms regulating its transcription, mutated Cdc14 promoters plus chimeras of selected Cdc14 sequences and a minimal promoter were tested in stable transformants. This revealed that a tandem repeat o...

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Internuclear gene silencing in Phytophthora infestans.

Transformation of the diploid oomycete plant pathogen Phytophthora infestans with antisense, sense, and promoter-less constructs of the coding sequence of the elicitin gene inf1 resulted in transcriptional silencing of both the transgenes and the endogenous gene. Since heterokaryons obtained by somatic fusion of an inf1-silenced transgenic strain and a wild-type strain displayed stable gene sil...

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Accumulation and Resistance to Phytophthora infestans

Active oxygen species (AOS) are responsible for triggering defense responses in plants. Respiratory burst oxidase homologs ( rboh genes) have been implicated in AOS generation. We have isolated two rboh cDNAs, NbrbohA and NbrbohB , from Nicotiana benthamiana leaves. NbrbohA was expressed constitutively at a low level and the transcripts were increased after mechanical stress of control leaf inf...

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ژورنال

عنوان ژورنال: Virus Research

سال: 2019

ISSN: 0168-1702

DOI: 10.1016/j.virusres.2019.197674